[Pavel Chaloupka]

[quote=”Vale!” post=3760]So is there an optimum duration that you’d allow a culture to run ; and how do you tell if a culture requires renewal ‘early’, as it were?[/quote] Sorry, did not answer this. This is the hard part that you need to learn from experience, so it is best to run several cultures and keep them on different densities. The same with algae. It is very dependent of how much and what you feed it (and as well as with the fish, you need to learn to feed just enough dependent on the density), the atributes of the starting culture (some populations will be able to survive under worse conditions then others) That is why it is best to collect for example Rotifera inoculations from muddy puddles. Lab Moina cultures may be much more sensitive to polution than some wild Moina you would collect yourself and vice versa.